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Reconstitution Guide

How to reconstitute

A simple step-by-step for preparing lyophilised peptides in the laboratory. For research use only — not for human consumption.

1

Prepare your area

Wash your hands thoroughly and clean your work surface. Work in a clean, draught-free space.

2

Sanitise the stoppers

Wipe the rubber stoppers of both the peptide vial and the bacteriostatic water with an alcohol swab, and let them air-dry.

3

Draw bacteriostatic water

Pull air into the syringe equal to the water you'll draw, inject it into the water vial, then invert and slowly draw your measured volume (e.g. 2 mL for a 20 mg vial).

4

Add water to the peptide

Insert the needle into the peptide vial and run the water slowly down the inside glass wall — never directly onto the powder. This prevents foaming and protects the peptide.

5

Dissolve gently

Do NOT shake. Gently swirl or roll the vial; most peptides dissolve within a few minutes. Avoid heat and direct sunlight.

6

Store correctly

Keep the reconstituted vial refrigerated (2–8 °C) and protected from light. Discard if the solution is cloudy, discoloured or contains particles.

Calculate concentration

Concentration = total peptide (mg) ÷ total water (mL).

20 mg ÷ 2 mL = 10 mg/mL

So 1 mL = 10 mg, and 0.1 mL = 1 mg.

Important notes

  • ✓ Use sterile technique throughout.
  • ✓ Always reconstitute with bacteriostatic or sterile water — never tap water.
  • ✓ Store reconstituted vials refrigerated and respect each peptide's stability window.
  • ✓ Discard if cloudy, discoloured or containing particles.
  • ✓ A Certificate of Analysis is available on request.

Reconstitution & concentration calculator

Reconstitution and concentration calculator - research use only

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