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A simple step-by-step for preparing lyophilised peptides in the laboratory. For research use only — not for human consumption.
Wash your hands thoroughly and clean your work surface. Work in a clean, draught-free space.
Wipe the rubber stoppers of both the peptide vial and the bacteriostatic water with an alcohol swab, and let them air-dry.
Pull air into the syringe equal to the water you'll draw, inject it into the water vial, then invert and slowly draw your measured volume (e.g. 2 mL for a 20 mg vial).
Insert the needle into the peptide vial and run the water slowly down the inside glass wall — never directly onto the powder. This prevents foaming and protects the peptide.
Do NOT shake. Gently swirl or roll the vial; most peptides dissolve within a few minutes. Avoid heat and direct sunlight.
Keep the reconstituted vial refrigerated (2–8 °C) and protected from light. Discard if the solution is cloudy, discoloured or contains particles.
Concentration = total peptide (mg) ÷ total water (mL).
So 1 mL = 10 mg, and 0.1 mL = 1 mg.






